Method for obtaining of glucose isomerase

摘要

A method for producing glucose isomerase comprising the steps of cultivating the enzyme producing strain Streptomyces sp. 1339 registration N. 144 Bulgarian State Institute for Drug Control, for 36 to 72 hours in a culture medium with xylose as an indicator, the temperature being kept at 24 DEG to 36 DEG C., the initial pH of cultivation being from 6.5 to 9.0 and effective isomerization at a temperature of 50 DEG to 80 DEG C., and a pH of 6.0 to 9.0 in the presence of 1x10-4M CoCl2.6H2O 1x10-2M MgSO4.7H2O in 0.1 to 3 M of the substrate.