| 摘要 |
<p>PURPOSE:To obtain a tissue like saffron stigma and to produce a valuable component of saffron from the stigma-like tissue in high efficiency, by carrying out tissue culture of style and/or ovary of saffron in a medium containing a specific substance. CONSTITUTION:Style and/or ovary of saffron are taken out before blooming, i.e. in the state of a bud. The tissue is placed on a medium such as Murashige- Skoog medium added with 5-15ppm of benzyl-adenine and 0.1-10ppm of alpha- naphthaleneacetic acid and cultured for 1-2 months in dark place at 25 deg.C to effect the production and growth of a stigma-like tissue. The stigma-like tissue is optionally ground and is extracted with a solvent such as water, hydrous ethanol and hydrous propylene glycol at normal temperature or under heating to obtain a saffron pigment.</p> |
