| 发明名称 |
VERFAHREN ZUR RÜCKFALTUNG VON PROTEINEN MIT FREIEN CYSTEINRESTEN |
| 摘要 |
The present invention relates to novel methods for making and refolding insoluble or aggregated proteins having free cysteines in which a host cell expressing the protein is exposed to a cysteine blocking agent. The soluble, refolded proteins produced by the novel methods can then be modified to increase their effectiveness. Such modifications include attaching a PEG moiety to form PEGylated proteins. |
| 申请公布号 |
DE60129432(T2) |
申请公布日期 |
2008.04.17 |
| 申请号 |
DE2001629432T |
申请日期 |
2001.05.16 |
| 申请人 |
BOLDER BIOTECHNOLOGY INC. |
发明人 |
ROSENDAHL, MARY S.;COX, GEORGE N.;DOHERTY, DANIEL H. |
| 分类号 |
C07K1/113;C12N15/09;C07K1/02;C07K14/00;C07K14/505;C07K14/515;C07K14/52;C07K14/535;C07K14/56;C07K14/61;C07K14/78;C12P21/02 |
主分类号 |
C07K1/113 |
| 代理机构 |
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代理人 |
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