MIKROBIOLOGISKT FRAMSTELLD POLYPEPTID MED SAMMA AMINOSYRASEKVENS SOM HOS PROINSULINET AV PRIMATER, DNA OCH PLASMIDER, VILKA KODIERAR FOR DENNA SEKVENS MIKROORGANISMER, SOM INNEHALLER DESSA GENETISKA INFORMATIONER, ...

摘要

The process for the preparation of the polypeptide which contains the amino-acid sequence of the proinsulin of primates entails the genetic information for the biosynthesis of the said polypeptide being obtained by means of mRNA from primates, the cDNA being prepared therefrom and inserted into a plasmid, the resulting hybrid plasmid being used to transform microorganisms, and the microorganisms producing the polypeptide being obtained by selection and cultivation.