摘要 |
A new glycoprotein is characterized by a protein consisting essentially of 75+/-6% of alpha -amino-acids, a carbohydrate portion of 24.6+/-5.2%, which contains 8.9+/-2% of hexoses, 7.1+/-1.5 of N-acetylated hexosamine, 0.2+/-0.2% of fucose, 8.4+/-1.5% of, N-acetylated neuraminic acid; a sedimentation coefficient S20w of 2.5+/-0.3 S; a molecular weight of 35,000+/-5,000, determined in the ultracentrifuge, and a molecular weight of 65,000+/-10,000, determined in polyacrylamide gel containing sodium dodecyl sulfate; an iso-electric point at pH 3.4+/-0.4; an extinction coefficient E11%cm (280 nm) of 1.9+/-0.3; an electrophoretic mobility in the range between that of alpha 1 and alpha 2-globulins; and a specific immunologic reaction with an antibody specifically directed against the glycoprotein.
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