PROCESS AND REAGENT FOR DETERMINATION OF URIC ACID

摘要

Uric acid in biological material is determined by means of uricase, catalase and aldehyde dehydrogenase in the presence of alcohol and nicotinamide-adenine-dinucleotide or phosphate (NAD(P), and at least one compound selected from oxalates, malonic acid mono(lower) alkyl esters, trihaloethanols, pyrazole and pyridine optionally substituted with one or more lower alkyl radicals and/or halogen atoms, pyridine-carboxylic acids optionally substituted by a lower alkyl radical and the amides and lower alkyl esters thereof, thiourea, isobutyramide and chelate-forming complexing agents; in this way there is provided a disturbance-free determination of uric acid in, for example, serum and plasma, which may be important in, for example, the clinical investigation of gout.