发明名称 |
Process for purification of crude kallikrein |
摘要 |
A process for purification of crude kallikrein utilizing a specific affinity of kallikrein to p-aminobenzamidine, which comprises bringing a solution, containing kallikrein and contaminated by other undesirable enzymes and proteins, into contact with a water-insoluble carrier combined with p-aminobenzamidine, exclusively eluting out the other enzymes and proteins from the carrier by the use of a buffer solution of lower NaCl concentration and then exclusively eluting out kallikrein by the use of a buffer solution of higher NaCl concentration.
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申请公布号 |
US4252902(A) |
申请公布日期 |
1981.02.24 |
申请号 |
US19790058292 |
申请日期 |
1979.07.17 |
申请人 |
TOHO PHARMACEUTICAL INDUSTRIES CO., LTD. |
发明人 |
FUJII, SETSURO;NODA, KIYOAKI;YOKOSHIMA, AKIO |
分类号 |
A61K35/39;A61K38/48;C07G99/00;C12N9/64;(IPC1-7):C12N9/50;C12N9/94 |
主分类号 |
A61K35/39 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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