ARTIFICIAL CHROMOSOME

摘要

An artificial mammalian chromosome, more specifically, a clone containing a mammalian centromere sequence and a DNA replication origin and having mammalian telomere sequences added to both ends is provided by preparing a library of CEPH artificial yeast chromosomes containing a human genome inserted thereinto, identifying clones holding a repetitive human alphoid sequence from this library, and further preparing a yeast strain which adds mammalian telomere sequences to the ends of the chromosome.