Method and apparatus for isoelectric focusing without use of carrier ampholytes

摘要

An ampholyte is purified by being enclosed in a capillary system forming a convection-free zone, direct current being passed through said zone. The electrolyte contains at least one buffering ion constituent. Electrolyte zones are created on both sides of said convection-free zone. Convection is created in said electrolyte zones to homogenize the electrolyte in said zones. Two equal liquid flows of reverse directions are created between said two homogenized electrolyte zones.