Method for isolating MB creatine kinase

摘要

Creatine kinase MB isoenzyme can be separated from a mixture of creatine kinase isoenzymes by ion exchange chromatography. Use of a mixture of cation and anion exchange resin in this method permits elution of the MB isoenzyme from the mixed resin while the other isoenzymes are wholely or partially retained. In a preferred embodiment the mixed resin is a mixture of a weakly basic anion exchange resin and a weakly acidic cation exchange resin.