| 摘要 |
Biologically active proteins are immobilized onto a carrier by reacting a compound containing at least two secondary amino groups at a temperature of from -80 DEG to +50 DEG C with a chlorination agent with the conversion of the secondary amino groups into iminochloride groups of the formula <IMAGE> in which R and R1, which can be the same or different, are aliphatic, aromatic or aliphatic-aromatic radicals, and reacting the carrier material product thus obtained (A) DIRECTLY WITH A BIOLOGICALLY-ACTIVE PROTEIN, IN AN AQUEOUS SOLVENT, OR (B) WITH A BIFUNCTIONAL COMPOUND CONTAINING AT LEAST ONE PRIMARY AMINO GROUP, OPTIONALLY IN AN AQUEOUS OR ORGANIC SOLVENT, AND REACTING THE PRODUCT THEREBY OBTAINED CONTAINING GROUPS OF THE FORMULA <IMAGE> in which R2 is an alkylene radical, an alpha , omega -dioxyalkylene radical or a dicarboxylic acid amide group containing 2 to 8 carbon atoms and X is a primary amino group or a function capable of protein binding either (I) DIRECTLY WITH A PROTEIN OR (II) FIRST WITH AT LEAST ONE BIFUNCTIONAL OR POLYFUNCTIONAL PROTEIN REAGENT AND THEREAFTER WITH A PROTEIN OR WITH A REACTION PRODUCT OF A PROTEIN WITH THE PROTEIN REAGENT. |
