| 摘要 |
<p>A method of plant transformation whereby a regenerated transgenic plant containing desired genes introduced thereinto is prepared at a high efficiency and which permits the preparation in the subsequent generation of a transgenic plant containing the desired genes but not the drug-resistant genes used as the selection marker. The method comprises transforming a plant by means of agrobacteria and is characterized by cotransforming plant cells with a first T-DNA(1) which functions in plants and contains the drug-resistant genes and a second T-DNA(2) which is contained in the following hybrid vector and contains, inserted therein, desired DNA fragments to be introduced into the plant. The hybrid vector is one prepared by the homologous recombination between the following acceptor vector and the following intermediate vector in agrobacteria. The acceptor vector contains at least (a) a DNA region having the function of plasmid replication which is effective in agrobacteria and colibacilli, (b) a DNA region containing vir B and vir G genes of the virulent region of Ti plasmid pTiBo542 of Agrobacterium tumefaciens, and (c) a DNA region which is homologous with part of the following intermediate vector and is capable of homologous recombination via that part in agrobacteria. The intermediate vector contains at least (i) a DNA region having the function of plasmid replication which is effective in colibacilli but ineffective in agrobacteria, (ii) a DNA region which is homologous with part of the above acceptor vector and is capable of homologous recombination via that part in agrobacteria, and (iii) a DNA region which constitutes at least part of the second T-DNA(2).</p> |
