| 摘要 |
Electrophoresis apparatus including a cooperating gel slab and matrix member. The matrix member has a first surface in generally surface-to-surface contact with the gel and is positioned overlying the gel. The matrix member has a plurality of openings therein which cooperate with the gel member to define a series of upwardly open wells. Electrode means pass through the wells. A reservoir for providing buffer solution to the gel is provided in underlying relationship with respect to the matrix member-gel slab assembly. The matrix member-gel slab assembly are urged into intimate contact. In a preferred form, integrally formed channel means cooperate with the underlying slab and a pump to establish a vacuum which serves to urge the matrix member-gel slab into intimate contact. The reservoir may have a support bracket for securing the matrix member-gel slab in overlying relationship to a buffer solution containing trough so that the matrix member-gel slab is maintained in generally horizontal position. In this fashion the material to be separated, such as proteins, migrates upward from inside the gel into the overlying wells. A plurality of molecules separated previously as by a standard slap gel system, for example, are eluted into separate wells.
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