发明名称 |
Conditional homologous recombination of large genomic vector inserts |
摘要 |
An efficient high throughput method for modifying genes in a recombination deficient host cell is disclosed. Such modifications include generating insertions, deletions, substitutions, and/or point mutations at any chosen site in the independent origin based cloning vector. The modified gene is contained in an independent origin based cloning vector that is used to introduce a modified heterologous gene into a cell. Such a modified vector may be used in the production of a germline transmitted transgenic animal, or in gene targeting protocols in eukaryotic cells. |
申请公布号 |
AU6229800(A) |
申请公布日期 |
2001.02.05 |
申请号 |
AU20000062298 |
申请日期 |
2000.07.20 |
申请人 |
THE ROCKEFELLER UNIVERSITY |
发明人 |
NATHANIEL HEINTZ;XIANGDONG W. YANG;SHIAOCHING GONG |
分类号 |
C12N15/10;C12N15/64;C12N15/65;C12N15/90 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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