SPECIFIC SORBENTS AND A METHOD OF THEIR PREPARATION

摘要

The invention is concerned with Specific sorbents based on hydrophylic polymeric gels, preferably or a macroporous character, which carry covalently bonded D-aminoacid or a peptide containing D-aminoacid units. The basis carrier which is prepared by copolymerization of hydroxyalkyl esters of hydroxylalkylamides of acrylic or methacrylic acid with crosslinking acrylate or methacrylate comonomers is modified by reaction with diamines, aminoacids or dicarboxylic acids and the resulting carboxyterminal or aminoterminal groups are condensed with D-analogs of aminoacids or peptides. The peptide containing D-aminoacids can be also stepwise synthetized on the surface of the carrier. The specific sorbents of the invention are used as insolubilized inhibitors in the affinity chromatography of proteolytic enzymes. They do not undergo an irreversible inactivation during repeated application and are suitable for technological purpose because of their mechanical and hydrolytical stability.