| 摘要 |
NEW MATERIAL:The title substance characterized by: molecular weight 27,000 + or - 1,000; peak of UV-abasorption specturm, 275-280 mmu, IR absorption spectrum, characteristic absorptions of protein; isoelectric point, about pH7; soluble in water and aqueous solution of NaCl having an ion strength of 0.01-0.1; optimum pH range for protease activity, >=8,5; that for esterase activity, >=7.5; that for amidase activity, about 8.5; optimum temperature for protease activity, about 50 deg.C; stable at pH5-12 at a low temperature; stable by heating at 40 deg.C and pH9.5 for 60 min; more than 90% of the activity is lost by heating at 70 deg.C for 60 min; selectively and specifically hydrolyzes the ester bond and amide bond of carboxyl group in L-lysine; inhibited by diisopropyl phosphofluoride, etc. USE:Selective and specific hydrolysis of ester bond and amide bond of carboxyl group in L-lysine. PROCESS:Culturing of Achromobacter (e.g. Achromobacter lyticus M497-1). |
