| 摘要 |
<p>The present invention encompasses a method for stabilizing interferon during purification comprising treating an interferon preparation with an effective stabilizing amount of reduced glutathione, thiodiglycol, thioethanolamine, thioalkanoic acid having 1-7 carbon atoms, monothioglycerol, dithiotreitol, thiotic acid, N-acetylcysteine, or N-acetylhomocysteine as a sulfur containing reducing agent for selectively retaining interferon sulfhydryl groups in the reduced state. The method of the present invention preserves the activity of interferon by selectively retaining interferon sulfhydryl (-SH) functions in the reduced state without reducing interferon disulfide linkages (-S-S-) which are essential for activity.</p> |
