摘要 |
PURPOSE:To simply and rapidly analyze paracort in serum by, eluting paracort in serum by a high performance liquid chromatograph and converting said paracort to a paracort radical before detecting the same. CONSTITUTION:When an apparatus is operated to inject a serum specimen preliminarily subjected to deproteinizing treatment in a column 1, paracort contained in the specimen is separated from other impurities and discharged to a mixing coil as a paracort ion while the paracort ion receives the action of sodium hydrosulfide from a specimen tank 5 to form a paracort radical. Thus formed paracort radical is specifically detected by a visible light absorptiometer 7 and a signal with a level proportional to the concn. thereof is outputted. |