| 摘要 |
<p>A diagnostic test system for detecting .beta.-galactosidase producing micro-organisms is prepared by impregnating one æone of the bibulous material with an aqueous acetone solution of an o-nitro-phenyl-.beta.-D-galactopyranoside substrate; and impregnating a contiguous zone of the bibulous material with a solution of a micro-biologically inert buffer system which will maintain a pH of from about 6.5 to 8.5. Preferably, the bibulous material contains 1.5 mg of dry o-nitrophenyl-.beta.-D-galactopyranoside and 0.02 millimoles of a buffer which will maintain a pH of 7.4. Optionally, a waterproof barrier zone and an identifying dye zone may be present. In use, the impregnated bibulous material is inserted into a test tube of a saline suspension of unknown organism and incubated for four hours. The development of a yellow coloration indicates that .beta.-galactosidase is produced by the organism being tested. The diagnostic test system of this invention is stable for one year at 4.degree.C,, at room temperature, and at 37.degree.C.</p> |
