| 摘要 |
<p>PURPOSE:To stably multiply a plant of the genus Iris without being influenced by natural environment by inoculating a cut piece of the root part of leaf of a plant of the genus Iris in a solid medium containing an auxin. CONSTITUTION:The root part of leaf of a plant of the genus Iris is used as a cut piece of perfume plant, sterilized, inoculated into a solid medium for tissue culture of plant, containing an auxin and optionally a cytokinin and a callus-like tissue is derived. A plant of the genus Iris is an important perfume plant to accumulate an oris oil, a kind of high-quality natural purified oil in the rhizome part thereof. The cut piece is inoculated as mentioned above to derive a callus. When the callus is subjected to subculture in a medium containing an auxin, regeneration ability to plant can be maintained for a long period of time. Further the callus is transplanted into a given regeneration such as a medium not containing a plant hormone or a medium containing a cytokinin and a plant can be regenerated.</p> |
