| 摘要 |
1533537 Enzyme preparations A NATTERMANN & CIE GmbH 5 May 1977 [7 May 1976 15 April 1977] 18930/77 Heading C3H In a process of manufacturing enzyme preparations rich in lipase from pancreas tissue, the comminuted pancreas tissue is mixed in an organic solvent mixture consisting of 80-95 volume per cent of halogenated hydrocarbon, whose boiling point at normal pressure is from 47‹ to 130‹ C. and whose density amounts to at least 1À3 or aliphatic and/or aromatic hydrocarbon whose boiling point at normal pressure is from 47‹ to 130‹ C. and whose density is from 0À655 to 0À88, together with 5-20 volume per cent of an alcohol having 4-6 C atoms which is substantially immiscible with water or a mixture of said alcohol and acetone, the solvent mixture is allowed to exert its effect on the tissue at at least 10‹ C., the obtained tissue-water containing emulsion phase is separated from the other phase or phases and a dry enzyme-preparation is obtained from said emulsion phase. Preferably the organic solvent mixture consists of a halogenated hydrocarbon and the alcohol or the alcohol acetone mixture, and is allowed to exert its effect on the tissue for 12-40 hours at 15‹ to 20‹ C. After about 12-15 hour action of the solvent mixture, the lower fat-containing solvent phase may be removed and fresh solvent mixture is added again, then the material is stirred for about 1 hour at 20-35‹ C., and is then allowed to settle prior to the processing of the central emulsion phase. |
