| 摘要 |
1532057 Centrifugal separators F HOFFMANN-LA ROCHE & CO AG 6 Jan 1976 [28 Feb 1975] 01825/76 Heading B2P [Also in Divisions Gl and G2] An analysis process comprises the following steps:-(a) a specimen for analysis is mixed with one or more reagents in a vessel having a target portion of progressively decreasing cross-section; (b) the mixture is incubated; (c) the incubated mixture is then centrifuged so as to hurl agglutinates to the narrow end of the target portion which is disposed in the direction of the centrifugal force with the result that the agglutinates stick to the solid narrow end whereas unagglutinated elements remain free thereon, whereafter (d) centrifuging is stopped and the nature of the reaction which has occurred is determined by observing the presence or absence of agglutinated or non agglutinated elements after a time sufficient for their separation. The process may be directed to an analysis of blood and in this instance the blood is first separated into globules and plasma such as by centrifuging. The globule specimen to be analyzed may be treated with an enzyme e.g. bromelin to accelerate the agglutination or dumping. Before the mixing step, the vessel is suitably covered internally with a thin layer of proteins e.g. ox-blood serum albumins. To facilitate mixing, the vessel may be vibrated in a horizontal or vertical plane. A suitable vessel 1 is shown in Fig. 1 secured to a support 2 and rotatable about centrifugal axis Z. The support may be in the form of a ring on a disc carrying a number of vessels. The vessel is provided with an aperture 3 for the introduction of specimen and reagents and on centrifugation agglutinated particles will be hurled to position 7 the point of narrowest cross section. The nature of the reaction occurring in the vessel may be determined automatically by means comprising at least a microscope and a photoelectric cell (Figs. 9 and 22, not shown). Alternatively, the specimen or reagent is radioactive and the nature of the reaction may be determined by means of a radioactivity counter. |
