| 摘要 |
1527268 Antiserum comprising immunoglobulin-G BIOTEST SERUM INSTITUT GmbH 16 July 1976 [18 July 1975] 29689/76 Heading C3H [Also in Division G1] An antiserum for the quantitative determination of one or more of the degradation products X and Y of fibrin and fibrinogen obtained by the action of plasma on fibrin or fibrinogen: may be prepared by a process which comprises immunization of one or more experimental animals with the purified degradation product(s), and repeatedly adsorbing the antiserum on adsorbents until the antiserum no longer reacts with plasma, serum or fibrinogen, but only with the degradation products X and Y of fibrin and fibrinogen when tested by Ouchterlong's immune diffusion technique. The adsorption may be effected with heparin plasma, citrate plasma, oxalate plasma or ethylenediaminetetraacetic acid plasma, which plasmas may be cross-linked with glutaraldehyde. The antiserum may be treated with plasma coupled to agarose gel which gel has been treated with cyanogen bromide. The immunoglobulin-G fraction may be isolated by fractionation of the specific antiserum, preferably on a cross-linked polysaccharide. |
