摘要 |
1513104 Bacterial polysaccharides comprising mannuronic and guluronic acid residues TATE & LYLE Ltd 18 May 1977 [28 May 1976] 22318/76 Addition to 1331771 Heading C3U A process for the production of a polysaccharide consisting of a partially acetylated variable block copolymer of 1-4 linked D- mannuronic and L-guluronic acid residues comprises subjecting to continuous cultivation a bacterium of the species Azotobacter vinelandii, preferably NICB 9068, 8789 or 8660, under aerobic conditions in an aqueous culture medium containing as essential ingredients at least one monosaccharide or disaccharide as carbon source and sources of phosphate, molybdenum, iron, magnesium, potassium, sodium, calcium and sulphate, the medium containing a fixed source of nitrogen and/or the aerating gas containing nitrogen, the concentration of the phosphate in the medium being limiting on the concentration of the bacteria and being at least 1À0 millimolar, and during the cultivation the pH being 6À0-8À2. |