Process and reagent for the rapid quantitative determination of lactate or alanine

摘要

L-lactate is quantitatively determined by the reaction thereof with nicotinamide-adenine-dinucleotide (NAD) in the presence of lactate dehydrogenase (LDH) with the formation of reduced NAD (NADH) and pyruvate, reaction of the latter with glutamate in the presence of glutamate-pyruvate-transaminase (GPT) with the formation of alanine and alpha -ketoglutarate in alkaline buffered solution and measurement of the NADH formed, wherein the reaction is carried out in the presence of at least 0.4 mol/liter of carbonate or bicarbonate.