DEVICE FOR ISOLATING MICROBIAL PATHOGENS

摘要

A mixing and centrifugation device particularly suitable for use in procedures involving detection of microbial pathogens which includes an elongated enclosed centrifugation receptacle containing an evacuated space and having an injectable closure on an end thereof with an enclosed treating fluid chamber disposed in communication and adjacent the injectable closure and separated from the evacuated space by a thin rupturable membrane. A sample treating fluid is carried within the enclosed treating fluid chamber and a sterile aqueous solution having a greater density than a sample fluid but able to selectively receive microbial pathogens from the sample fluid is disposed within the evacuated chamber. A sample is deposited on the sterile aqueous solution via an injection needle by passing the injection needle through the injectable closure, the enclosed treating fluid chamber, and rupturing the thin membrane such that the sample treating fluid contacts the sample as it passes from the injection needle and is deposited on the liquid filter medium.