| 摘要 |
1488534 Preparation of arteries SOLCO BASEL AG 31 Oct 1975 [11 Nov 1974] 45405/75 Heading A2D A process for the preparation of arteries for use in heterologous transplants comprises obtaining a length of animal artery free from surrounding tissue, any collateral vessels being sealed off, removing muscle tissue and elastic fibres from the wall of the artery by proteolytic splitting whereby a solution containing a proteolytic enzyme at a pH at which said enzyme is effective is passed continuously through the artery and subjecting the collagen tube thereby produced to tanning by passing continuously therethrough a buffered crosslinking solution whereby the density and firmness of the tube is increased. The artery is preferably a carotid artery of bovine, porcine or ovine origin, suitably obtained from a young animal. The enzyme is preferably ficin, trypsin or papain and the tanning solution suitably contains dialdehyde starch, glyoxal, polyacrolein, formaldehyde, dialdehyde cellulose or glutaraldehyde. The density and firmness of the tubes are increased to such an extent that the tubes should be able to withstand a pressure of 240 mm Hg gauge. The method may be carried out in the apparatus shown in the accompanying Figure where the appropriate proteolytic enzyme-containing solution and the tanning solution are consecutively and continuously passed through arteries 1 by means of the circulating means consisting of the cylinder 8. tube 10, pump 11, reservoir 5 and tubes 2. |
