Hesperetin dihydrochalcone glucoside sweetener prepn. - by hesperidin dihydrochalcone degradation using carrier-bonded glycolytic enzyme system

摘要

Hesperetine dihydrochalcone glucoside, used as sweetener, is prepd. from hesperidin dihydrochalcone by using a carrier-bonded enzyme or enzyme system, pref. hesperidinase, having inhibited beta-glucosidase activity. Fixation to a carrier selectively inhibits beta-glucosidase activity without loss in rhamnosidase activity. Carrier may be an (in)org. polymer, e.g. cellulose, polyamides, but pref. activated silicate-contg. materials, esp. porous glass which is activated with a layer of diazotised aromatic diamine, or 4,4'-diaminodiphenylmethane.