| 摘要 |
A live vaccine against vesical eruptions in cattle from infectious pustular vulvovaginitis (IPV, = bovine vesicular coital exanthema) and used in the treatment of diseases of cattle arising from viruses of infectious bovine rhinotracheitis (IBR) and IPV and which is harmless and of universal applicaton is prepd. by tissue culture passages of an electrophoretically homogeneous virus population from an IPV source. The vaccine may be prepd. from any bovine animal tissue; specially suitable are calf kidneys, adrenals and epididymis. Culture is at 37-39 degrees in a suitable medium and after the appearance of the cytopathogenic effect as shown by 80-100 degrees destruction of the cell culture (about 24-48 hrs.) the centrifuged supernatant may be used direct as a vaccine or after lyophilisation and re-solution.
|
