PRODUCTION OF L-ASPARTIC ACID

摘要

NEW MATERIAL:The microbial strain belonging to Escherichia genus, and containing a hybrid plasma obtained by integrating a plasmid with deoxyribonucleic acid carrying the gene information of aspartase separated from a microbial strain belonging to Escherichia genus. USE:Production of L-aspartic acid in high yield. The cells of said microbial strain, the cultured product or treated cells are made to react with fumaric acid and ammonia or with ammonium fumarate to obtain L-aspartic acid. PREPARATION:The chromosome DNA is separated from the cell of micro-organisms belonging to Escherichia genus and having aspartase activity. The chromosome DNA is connected to a plasmid by conventional method. The resultant hybrid plasmid is integrated in the host microorganism. The transformed strain is cultured and divided to obtain the objective microbial strain.