CLOSTRIDIUM DIFFICILE TARGETING MOIETIES AND CONSTRUCTS COMPRISING SAID MOIETIES

摘要

In certain embodiments, constructs are provided that selectively/preferentially inhibit and/or kill Clostridium difficile. In certain embodiments the constructs comprise a peptide that binds C. difficile attached directly or through an amino acid or a linker to an antimicrobial peptide.

主权项

1. A targeting peptide that binds to Clostridium difficile, where said peptide comprises or consists of an amino acid sequence selected from the group consisting of:
VPAKLLRVIDEIP (SEQ ID NO:3) where said peptide does not comprise the amino acid sequence VPAKLLRVIDEIPE (SEQ ID NO:2); VPAKLLRVIKKIP (SEQ ID NO:4), VPAKLLRVIKEIP (SEQ ID NO:5), NILRVLKQVWK (SEQ ID NO:20), GNFYRLFKDILK (SEQ ID NO:28); a fragment of VPAKLLRVIDEIP (SEQ ID NO:3) comprising at least 6, or at least 8, or at least 10 contiguous residues of said sequence where said peptide does not comprise the amino acid sequence VPAKLLRVIDEIPE (SEQ ID NO:2); a fragment of VPAKLLRVIKKIP (SEQ ID NO:4) comprising at least 6, or at least 8, or at least 10 contiguous residues of said sequence; a fragment of NILRVLKQVWK (SEQ ID NO:20) comprising at comprising at least 6, or at least 8, or at least 10 contiguous residues of said sequence; a fragment of GNFYRLFKDILK (SEQ ID NO:28) comprising at least 6, or at least 8, or at least 10 contiguous residues of said sequence; an inverso form of any of the preceding amino acid sequences; and an amino acid sequence that has at least 90% sequence identity with an amino acid sequence selected from the group consisting of VPAKLLRVIDEIP (SEQ ID NO:3), VPAKLLRVIKKIP (SEQ ID NO:4), VPAKLLRVIKEIP (SEQ ID NO:5), NILRVLKQVWK (SEQ ID NO:20), and GNFYRLFKDILK (SEQ ID NO:28), and where said sequence does not contain the amino acid sequence VPAKLLRVIDEIPE (SEQ ID NO:2); wherein said targeting peptide binds to C. difficile.