Muscle tissue regeneration using muscle fiber fragments

摘要

The invention is directed to methods and compositions for obtaining uniform sized muscle fiber fragments for transplantation. These muscle fiber fragments are able to reconstitute into long fibers that are oriented along native muscle. The implanted muscle cells integrate with native vascular and neural network, as confirmed by histology and immunohistochemistry. This invention is particularly advantageous because autologous muscle can be harvested from a donor site, processed and injected into target sites in the operating room. The fragmented muscle fibers can be readily integrated within the host.

主权项

1. A composition comprising:
a population of muscle fiber fragments that are capable of reconstructing elongated muscle fibers from the fragments, and a scaffold, wherein the fragments exhibit cell wall rupture and have an average size of less than 100 μm, and further wherein the composition further includes viable satellite cells associated with at least some of the fragments, wherein the muscle fiber fragments are incorporated into the scaffold and wherein the scaffold is an injectable scaffold selected from the group of collagen gel, fibrin gel, alginate gel, or UV-induced crosslinkable gel system.