Nitrilofor adsorbent för separation och immobilisering av proteiner, sätt att framställa adsorbentet, samt dess användning för fraktionering och immobilisering av biopolymerer

摘要

An adsorbent that comprises a solid phase for the isolation and immobilization of proteins by adsorption from a liquid phase is characterized in that it consists of a hydrophilic polymer network with covalently bound ligands of the formula - Y - X - R, in which R is an aliphatic substituent containing at least one nitrile group or a substituent having a heterocyclic ring system with at least one nitrogen atom in the ring and with one or more side groups consisting of or including -CN (nitrile group), where X is > NQ, >S, or >O, Q being H(CH2)n, where n = 0, 1, 2 or 3, and Y is an aliphatic side chain which joins R-X with the polymer network. The polymer preferably consists of agar or agarose in particle form having a particle size of <1 mm. Y can be -CH2-CH2SO2-CH2-CH2- and R can be The adsorbent can be used to isolate biopolymers and, inter alia, has been found highly effective in isolating proteins. The adsorbent can be produced by first activating the polymer with divinylsulphone and then adding the ligands.