Analyte detection via antibody-associated enzyme assay

摘要

The present invention provides methods, kits, and compositions for the detection of an analyte. The invention is particularly suited for the detection and quantification of an analyte in a sample. In the methods of the invention a complex is formed between an analyte specific binding agent and an analyte. The analyte specific agents are coupled to an enzyme possessing an activity that produces a PCR template indicative of the presence of the analyte. Amplification and detection of the PCR template yields a sensitive and quantitative measurement of analyte concentration.