Plants and seeds with altered storage compound levels, related constructs and methods involving genes encoding proteins with similarity to bacterial 2,4-dihydroxy-hept-2-ene-1,7-dioic acid class II-like aldolase proteins

摘要

This invention is in the field of plant molecular biology. More specifically, this invention pertains to isolated nucleic acid fragments encoding proteins with similarity to bacterial 2,4-dihydroxy-hept-2-ene-1,7-dioic acid class II-like aldolase proteins in plants and seeds and the use of such fragments to modulate expression of a gene encoding proteins with similarity to bacterial 2,4-dihydroxy-hept-2-ene-1,7-dioic acid class II-like aldolase proteins in a transformed host cell.

主权项

1. A transgenic plant comprising a suppression DNA construct, which is capable of suppressing expression of an aldolase-encoding gene, the construct comprising at least one heterologous regulatory element operably linked to:
(a) a polynucleotide encoding a polypeptide having an amino acid sequence of at least 95% sequence identity, based on the Clustal V method of alignment, when compared to SEQ ID NO: 31 or 49; or (b) a polynucleotide comprising at least 21 contiguous nucleotides of SEQ ID NO: 30 or 48; or (c) a full complement of the nucleic acid sequence of (a) or (b); wherein the suppression DNA construct is expressed in the plant to reduce endogenous aldolase activity and wherein seed of the plant has an increase in oil-content of at least 1%, on a dry-weight basis, when compared to a seed of a control plant not comprising said suppression DNA construct.