EARLY RECOGNITION PROCESS OF HPV-ASSOCIATED CARCINOMAS OR HIGH-GRADE, HPV-CAUSED DYSPLASIAS

摘要

A process for detecting mRNA that contains HPV-sequences and cellular sequences in an organic sample has the following steps: (a) collecting and preparing an organic sample; (b) isolating mRNA from the organic sample of step (a); (c) translating the thus isolated mRNA into cDNA with a usual primer for reverse transcription; (d) amplifying the thus obtained cDNA by a PCR reaction with a HPV-primer (5' primer) and a primer (3' primer) having sequences of the primer used in step (c); (e) splitting the amplified cDNA obtained in step (d) with an endonuclease that splits the 5' side of the HPV polyadenylating sequence; (f) amplifying the non-split cDNA of step (e) with the primers of step (d) or with nested primers; and (g) detecting the amplifying cDNA of step (f). Also disclosed is the use of such a process for the early recognition of HPV-associated carcinomas or high-grade, HPV-caused dysplasias.