Methods and kits used in the detection of fungus

摘要

The invention encompasses method of using quantitative PCR to detect fungal organisms in clinical and environmental samples to generate standards that allow the quantification of fungal organisms in the samples.

主权项

1. A method of detecting a fungus in a sample, the method comprising the steps of:
adding a first oligonucleotide mixture to an amplification mixture, wherein the first oligonucleotide mixture comprises oligonucleotides comprising sequences of SEQ ID NO. 6 and SEQ ID NO. 7 and the amplification mixture comprises a nucleic acid isolated from the sample; adding a second oligonucleotide mixture to the amplification mixture, wherein the second oligonucleotide mixture comprises at least two oligonucleotides selected from the group consisting of oligonucleotides comprising sequences of SEQ ID NO. 8, SEQ ID NO. 9, SEQ ID NO. 10, SEQ ID NO. 11, SEQ ID NO. 12, SEQ ID NO. 13, SEQ ID NO. 14, and SEQ ID NO. 15; subjecting the mixture to conditions that allow nucleic acid amplification; and detecting the presence of the fungus based on the results of the nucleic acid amplification.