E. COLI MEDIATED siRNA SILENCING OF AVIAN INFLUENZA IN CHICKENS

摘要

Product, and associated methods are described for the delivery of one or more small interfering RNAs (siRNAs) to an avian cell using a nonpathogenic bacterium for the prevention or treatment of Avian Influenza Virus (AIV). The siRNA is complementary to an mRNA of the AIV NP or PA sequence. In challenge studies with chickens, the siRNA is shown to prevent the onset of clinical symptoms or reduce the severity of disease, including reducing viral titers or virus shedding.

主权项

1. A nonpathogenic E. coli bacterium comprising a prokaryotic vector, said vector comprising a DNA molecule encoding one or more siRNAs and a promoter to control transcription of the siRNA, wherein the siRNAs interfere with one or more Avian Influenza viral RNA molecules and wherein the bacterium is engineered to express at least one inv gene and at least one hlyA gene.