| 主权项 |
1. A method for manufacturing an animal acellular tissue matrix material, comprising the steps of:
(1) collecting a raw material of an animal tissue, wherein the animal tissue is washed to remove blood and dirt, and cut into a tissue material having a length, a width and a height of the desired specification and dimension, and then the tissue material is preserved at a low temperature; (2) thawing and rehydrating the tissue material; (3) disinfecting and sterilizing the tissue material in a moderate alkaline solution, wherein the tissue material is then rinsed with sterile pure water, and the pH of the tissue material is adjusted to be neutral; (4) decellularizing and washing the tissue material; (5) digesting DNA components of the animal tissue, wherein the animal tissue is then rinsed with a normal saline; (6) optionally, digesting an antigen of α-1,3-galactose residue, i.e. α-Gal antigen, of the animal tissue, wherein the animal tissue is then rinsed with a high concentration of a sodium chloride solution, and rinsed with a normal saline; (7) inactivating the viruses in the tissue material and rinsing the tissue material; (8) optionally, packaging and sealing the tissue material under an aseptic condition; (9) optionally, terminal sterilization treatment of the tissue material, to obtain the animal acellular tissue matrix material; wherein, an enzymatic method is used to remove cell components and α-Gal antigen and improve the flexibility of a scaffold. |
