<p>The present invention relates to a method for in vitro maturation of a human gamete by culturing an immature human gamete in a chemically defined cell culture medium. The human gamete can be a spermatocyte or an oocyte. The maturation end point is metaphase II. The advantage of the described medium is a synchronised cumulus-, cytoplasm-, and nuclear maturation completed within a period of 20 to 30 hours. The medium should preferably contain ATA (Aurin Tri Carboxylicacid) as an anti-apoptotic agent. The medium should preferably not contain BSA, HSA or other directly serum derived products or substances.</p>
