发明名称 |
A QUANTUM METHOD FOR FLUORESCENCE BACKGROUND REMOVAL IN DNA MELTING ANALYSIS |
摘要 |
A method of background removal from melting curves generated using a fluorescent dye is provided for analyzing a melting profile of a nucleic acid sample. The method comprises measuring the fluorescence of the nucleic acid sample as a function of temperature to produce a raw melting curve having a melting transition, the nucleic acid sample comprising a nucleic acid and a molecule that binds the nucleic acid to form a fluorescently detectable complex, the raw melting curve comprising a background fluorescence signal and a nucleic acid sample signal; and separating the background signal from the nucleic acid sample signal by use of a quantum algorithm to generate a corrected melting curve, the corrected melting curve comprising the nucleic acid sample signal. |
申请公布号 |
US2016202195(A1) |
申请公布日期 |
2016.07.14 |
申请号 |
US201414914996 |
申请日期 |
2014.08.29 |
申请人 |
UNIVERSITY OF UTAH RESEARCH FOUNDATION |
发明人 |
Wittwer Carl T.;Sanford Lindsay N. |
分类号 |
G01N25/04;G01N21/64;C12Q1/68 |
主分类号 |
G01N25/04 |
代理机构 |
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代理人 |
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主权项 |
1. A method for analyzing a melting profile of a nucleic acid sample,
comprising measuring the fluorescence of the nucleic acid sample as a function of temperature to produce a raw melting curve having a melting transition, the nucleic acid sample comprising a nucleic acid and a molecule that binds the nucleic acid to form a fluorescently detectable complex, the raw melting curve comprising a background fluorescence signal and a nucleic acid sample signal; and separating the background signal from the nucleic acid sample signal by use of a quantum algorithm to generate a corrected melting curve, the corrected melting curve comprising the nucleic acid sample signal. |
地址 |
Salt Lake City UT US |