发明名称 |
Method for determination of direct bilirubin and reagent therefor |
摘要 |
<p>There is provided a method for determination of direct bilirubin which comprises the steps of contacting a bilirubin oxidase with a sample suspected of containing bilirubin; and, measuring direct bilirubin in the sample by optical changes of the sample, characterized in that bilirubin oxidase is allowed to act in the presence of a indirect bilirubin reaction inhibitor selected from a thiocyanate ion, a hydrazide, reduced nicotinamide adenine dinucleotide, reduced nicotinamide adenine dinucleotide phosphate and a potassium ion of 100 mM to 800 mM. By completely avoiding interference of indirect bilirubin, direct bilirubin is selectively and precisely determined using a reagent kit in the form of solution. The method and reagent of the invention are safe and free of environmental pollution due to unnecessary waste liquid treatment.</p> |
申请公布号 |
EP0882800(A2) |
申请公布日期 |
1998.12.09 |
申请号 |
EP19980110059 |
申请日期 |
1998.06.03 |
申请人 |
NITTO BOSEKI CO., LTD. |
发明人 |
KOJIMA, RYO;SASAGAWA, YOSHIKIYO;OKAZAKI, YASUNORI;NAGASAWA, TAKESHI |
分类号 |
C12Q1/26;(IPC1-7):C12Q1/26 |
主分类号 |
C12Q1/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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