METHOD FOR DETECTION, IDENTIFICATION AND COUNTING OF VIBRIO PARAHAEMOLYTICUS USING GENE (RPOD) SEQUENCE ENCODING RNA POLYMERASE SIGMA70 FACTOR

摘要

PROBLEM TO BE SOLVED: To produce a specific gene amplification primer having high performance and useful for the detection, determination and identification of Vibrio parahaemolyticus, having low risk of misjudgment and exhibiting practically sufficient amplification efficiency and amplification specificity. SOLUTION: The base sequences of an RNA polymerase σ70 factor rpoD gene of a standard strain of the genus Vibrio and a stock strain of Vibrio parahaemolyticus (a strain having toxin gene and a strain free from toxin gene) are determined, the system relationship of the strains is clarified and a characteristic base of Vibrio parahaemolyticus is identified to enable the design of a probe having high specificity and containing the base and a primer for the amplification of gene in high amplification efficiency and high specificity.