| 摘要 |
Provided is a bone regeneration agent comprising an inactivated cell construct derived from stem cells as a source material, the inactivated cell construct at least containing a mineral and an extracellular matrix. The bone regeneration agent has osteoconductive and osteoinductive abilities, and is inexpensively producible and size-controllable. Also provided is a method comprising the steps of:;(1) inducing differentiation of stem cells into mineral-producing cells in agitated culture under the condition of 0.01 to 1.00 Hz to give a cell aggregate at least containing a mineral and an extracellular matrix, or
(1′) inducing differentiation of stem cells genetically engineered to overexpress a protein associated with bone formation into mineral-producing cells in agitated or static culture, to give a cell aggregate at least containing a mineral and an extracellular matrix, and
(2) inactivating the cell aggregate obtained in the preceding step. The method enables easy production of the above-mentioned bone regeneration agent. |
