PRODUCTION OF PLANTS USING SOMATIC EMBRYOGENESIS

摘要

A process for propagating Theobroma cacao L. is described. The process uses direct or indirect somatic embryogenesis to produce primary embryos from explant material, and then applies direct somatic embryogenesis to the resulting primary embryos to produce secondary embryos. These may be matured, pre-germinated, and germinated to form plantlets. Plants, plants bearing fruit, and plant materials are also provided, as well as methods for processing the fruit of the plants to generate cocoa products.

主权项

1. A process for propagating Theobroma cacao comprising:
(a-1) obtaining Theobroma cacao L. explant material, followed by
(i) placing the explant material into an induction medium with exposure to light for a period sufficient to obtain primary embryos; and(ii) optionally transferring the primary embryos from (i) into a development medium and culturing them with exposure to light for a period sufficient to produce primary embryos by direct somatic embryogenesis; (b-1) removing tissue of primary embryos, followed by
(i) placing the tissue into an induction medium with exposure to light for a period sufficient to obtain secondary embryos; and(ii) optionally transferring the secondary embryos from (i) into a development medium and culturing them with exposure to light for a period sufficient to produce secondary embryos by direct somatic embryogenesis; (c) transferring the secondary embryos from step (b-1) to a medium configured to cause maturation of the secondary embryos.