| 摘要 |
The present invention relates to a method for constructing human skin tissue, in which human skin tissue is reconstructed on a body surface of a non-human animal, which includes: (a) burying a cell-injectable cylindrical chamber into a dorsal skin of an immunodeficient non-human animal, (b) injecting a cell suspension containing a cultured human fibroblast and a cultured human keratinocyte into the chamber, (c) cutting an upper bottom part of the chamber 7 or more days after grafting of the cell to fully open the cylinder part of the chamber, and then (d) eliminating the chamber. The present invention provides a reconstructed human skin which is excellent in terms of quantity and convenience for researching expression of genes and proteins of a human skin or evaluating medicines acting on a human skin, particularly melanocyte.
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