摘要 |
Provided is a qualitative and absolute quantification kit for detecting hepatitis B virus cccDNA. The kit comprises an HBV DNA extraction reagent, Plasmid-Safe TM ATP-Dependent DNase, an upstream primer with a DNA sequence of SEQ ID NO.1, a downstream primer with a DNA sequence of SEQ ID NO.2, a Taqman probe with a DNA sequence of SEQ ID NO.3, EvaGreen fluorescent dye, common PCR DNA polymerase, and digital PCR DNA polymerase. |