Device for guiding cell migration and method of guiding cell migration implementing such a device

摘要

A guiding device in which cells are confined between a support surface and a textured surface of a substrate, said textured surface having an anisotropic three-dimensional structure having a repeating pattern that repeats according to a repeat axis, said repeating pattern having a succession of guide spaces adjacent to one another according to the repeat axis, each of said guide spaces being capable of receiving at least one portion of one of the cells and being oriented according to a direction of anisotropy to guide a movement of the cells in the direction of anisotropy.

主权项

1. A device for guiding cell migration, comprising a support surface provided with cells, and a substrate, said substrate having a textured surface placed facing the support surface at a distance along a vertical direction, said textured surface being in contact with the cells placed on the support surface such that the cells are confined in the vertical direction between the support surface and the textured surface, said textured surface having a base surface and an anisotropic three-dimensional structure presenting a repeating pattern along a repeat axis perpendicular to the vertical direction, said repeating pattern having a succession of guide spaces adjacent to one another along the repeat axis, the anisotropic three-dimensional structure comprising a plurality of pairs of guide surfaces, said pairs of guide surfaces being adjacent to each other along the repeat axis and defining the repeating pattern, each pair comprising first and second guide surfaces which extend from the base surface facing one another and which define between them one of the guide spaces, each of said guide spaces being capable of receiving at least a portion of one of the cells and being oriented in a direction of anisotropy perpendicular to the vertical direction in order to guide movement of the cells in the direction of anisotropy.