A process for efficiently and economically producing a vinylated nucleic acid by subjecting an amino-containing nucleic acid to a vinylation reaction with a vinylating agent. A method of economically and efficiently producing a vinylated nucleic acid by a method which comprises performing PCR in the presence of an amino-modified nucleotide and dNTP to thereby introduce vinyl group into the amplified PCR product, or by a method which comprises performing PCR in the presence of a vinyl-modified nucleotide and dNTP.
申请公布号
WO02062817(A1)
申请公布日期
2002.08.15
申请号
WO2002JP01108
申请日期
2002.02.08
申请人
MITSUBISHI RAYON CO., LTD.;URAGAKI, TOSHITAKA;WATANABE, FUMIAKI;NAGAHAMA, CHIAKI;YU, FUJIO;KIKUCHI, KATSUAKI;YOSHIOKA, AKIRA