| 主权项 |
1. A method for preparation of one or more human milk oligosaccharides (HMOs) or derivatives or precursors thereof, the method comprising the steps of
a) providing at least one compound or a mixture of the compounds characterized by general formula 5 wherein R′ independently is fucosyl or H, with the proviso that at least oneR′ is fucosylR* is a group removable by hydrogenolysis or H, and optionally providing at least one compound or a mixture of compounds selected from the group consisting of:
optionally sialylated lactose derivatives of general formula 6 and salts thereof: whereinR* is a group removable by hydrogenolysis or H,R″ independently of each other is sialyl or H,lacto-N-tetraose (LNT): lacto-N-tetraose (LNT) derivatives of the following formula: wherein R is a group removable by hydrogenolysis;lacto-N-neotetraose (LNnT): lacto-N-neotetraose (LNnT) derivatives of the following formula: wherein R is a group removable by hydrogenolysis; b) adding at least one enzyme comprising a transglycosidase activity, at least one of which comprises a transfucosidase activity, to the at least one compound or a mixture of compounds provided according to step a); c) incubating the mixture obtained according to step b); d) optionally:
I. adding to the mixture obtained according to step c) at least one compound or a mixture of compounds selected from the group consisting of:
i. optionally sialylated and/or fucosylated lactose derivatives of general formula 2 and salts thereof: whereinR is a group removable by hydrogenolysis,R1 independently of each other is fucosyl or HR4 independently of each other is sialyl or H,provided that the compound of general formula 2 is not R-glycoside of lactose, if provided alone;ii. optionally sialylated and/or fucosylated lactose derivatives of general formula 4 and salts thereof: whereinR1 independently of each other is fucosyl or HR4 independently of each other is sialyl or H,provided that the compound of general formula 4 is not lactose, if provided alone;iii. lacto-N-tetraose (LNT): iv. lacto-N-tetraose (LNT) derivatives of the following formula: wherein R is a group removable by hydrogenolysis;v. lacto-N-neotetraose (LNnT): vi. lacto-N-neotetraose (LNnT) derivatives of the following formula: wherein R is a group removable by hydrogenolysis; andincubating the mixture obtained; and/orII. adding at least one enzyme comprising a transglycosidase activity to the mixture obtained according to step c) or step d)-I., and incubating the mixture obtained; and e) optionally subjecting the mixture obtained after step c) or step d) to a hydrogenolysis reaction. |
